Visn. Hark. nac. agrar. univ., Ser. Biol., 2021, Issue 2 (53), p. 61-70


A. P. Dascaliuc1, N. V. Zdioruk1, T. H. Ralea1, N. N. Jelev1, Yu. A. Pariy2, Ya. F. Pariy2

1Institute of Genetics, Physiology and Plant Protection

(Chisinau, Moldova)


2Ukrainian Scientific Institute of Plant Breeding

(Kyiv, Ukraine)


Experiments provided with the seeds of 50 wheat varieties reproduced in the Kharkiv region of Ukraine and Chisinau area of Moldova to elucidate the efficiency of rapidly assessing genotypes' primary resistance to high temperatures and frost. The tests were performed under laboratory-controlled conditions, based on the evaluation of the seeds' germination capacity after their exposition to shock with high or sub-zero temperatures. The obtained results demonstrated that by applying the elaborated methods, we could differentiate wheat genotypes by their primary resistance to extreme temperatures (excluding the adaptation processes induced during plant ontogenesis). Resistance of different wheat genotypes seeds to heat shock or shock with negative temperatures may vary, being influenced by the environmental conditions of their reproduction. The data obtained demonstrate that seeds resistance to both types of temperature shock is specific for different wheat varieties and can be influenced by conditions of seed reproduction. Due to this adaptive variability of genetic and epigenetic nature, wheat varieties and their descendants are characterized by high resistance and good productivity in different environmental conditions. The possibility of epigenetic inheritance suggests that it may influence the primary frost or heat resistance of wheat embryos. Because the meteorological conditions vary from year to year, they can influence the primary resistance of genotypes to heat stress factors even when seeds reproduced in the same zone. We consider that the assessment of the primary resistance of wheat genotypes offers new possibilities for determining its interference with other mechanisms of resistance of wheat genotypes to frost or heat. The elaborated methods are with perspectives for implementing in programs for selection or appreciation the heat or frost resistance of wheat genotypes.

Key words: Triticum aestivum, seeds, heat and frost tolerance, accelerated methods



1. Alexandrov V.Ya., Kislyuk I.M. 1994. Cell response to the heat-shock: physiological aspect. Cytology. 36 (1) : 5-59. (In Russian)
2. Zhuchenko A. A. 1988. The adaptive potential of cultivated plants: genetic and ecological bases. Publisher. Kishinev. Shtiintsa: 267 р. (In Russian)
3. Ivanov V.B. 2003. The problem of stem cells in plants. Russian Journal of Developmental Biology. 34 (4) : 205-212.
4. Udovenko G.V. 1988. Methodological guidance. Diagnosis of plants' resistance to stress. VIR, 228 р. (In Russian)
5. Jelev N. 2016. Diminishing the impact of abiotic stressors on Triticum aestivum L. plants by using the natural growth regulator Reglalg. Bulletin of the Academy of Sciences of Moldova. Life sciences. 3 (330) : 72-79. (In Romanian)
6. Blum A. 1996. Crop responses to drought and the interpretation of adaptation. Plant Growth Regul. 20 : 135-148.
7. Blum A. 1994. Stress tolerance in plants: what are we looking for? In: NATO ASI Series (Series H: Cell Biology). Springer, Berlin, Heidelberg. 86 : 315-324.
8. Clewer A.G, Scarisbrick D.H. 2001. Practical statistics and experimental design for plant crop science. Chichester, New York, 332 p.
9. Penfield S. 2017. Seed dormancy and germination. Curr. Biol. 27 (17) : R874-R878.
10. Dascaliuc A., Ivanova R., Arpentin Gh. 2013. Systemic approach in determining the role of bioactive compounds. In: Bioactive Compounds from Natural Sources for Prophylaxis and Treatment of the Effects of Radiological, Chemical and Biological Agents NATO. Science for Peace and Security Series A: Chemistry and Biology book series (NAPSA): 121-131.
11. Escobar L. A., Meeker W.Q. 2006. A Review of Accelerated Test Models. Statistical Science. 21 (4) : 552-577.
12. Fowler D. Byrns B. M., Greer K. J. 2014. Overwinter Low‐Temperature Responses of Cereals: Analyses and Simulation . Crop Sci. 54 : 2395-2405.
13. Jaligot E., Rival A. 2015. Applying epigenetics in plant breeding: balancing genome stability and phenotypic plasticity. In: Advances in Plant Breeding Strategies: Breeding, Biotechnology, and Molecular Tools. 1 : 159-192.
14. Levitt J. 1980. Responses of plant to environmental stresses. New York, 568 р.
15. Lopes M.S., Rebetzke G.J., Reynolds M.P. 2014. Integration of phenotyping and genetic platforms for a better understanding of wheat performance under drought. J. Exp. Bot. 659 (21) : 6167-6177.
16. Lopes M.S., Reynolds M.P. 2010. Partitioning of assimilates to deeper roots is associated with cooler canopies and increased yield under drought in wheat. Funct. Plant Biol. 37 : 147-156.
17. Novoseltsev V.I., Tarasov B.V., Golikov V.K., Demin B.E. 2006. Theoretical bases of the system analysis. Moscow, 592 p. (In Russian)