Visn. Hark. nac. agrar. univ., Ser. Biol., 2021, Issue 2 (53), p. 53-60


A. I. Kokorev

Dokuchaev Kharkiv National Agrarian University

(Kharkiv, Ukraine)


Polyamines are currently considered as stress metabolites of plants that have a direct protective effect on biomacromolecules and are also involved in cellular signal-regulatory processes. The most abundant diamine in plant cells is putrescine. At the same time, another diamine, cadaverine, was found in plants, in particular in cereals, the functions of which have been poorly studied. In this regard, we compared the effects of putrescine and cadaverine on the survival of wheat seedlings (Triticum aestivum L.) after damaging heating. We also studied the effect of these diamines on the content of hydrogen peroxide in roots of seedlings and the probable relationship between the induction of heat resistance of seedlings by diamines and changes in the redox homeostasis of cells. It was found that the pretreatment of seedling roots with both diamines at concentrations of 0.25–2 mM approximately equally increased survival after potentially lethal heat stress (heating in a water thermostat at 45.5°C for 10 min). The most noticeable protective effect of diamines was shown when they were used at a concentration of 1 mM. Under the influence of both putrescine and cadaverine, a transient increase in the content of hydrogen peroxide in roots was observed with a maximum after 2 hours from the start of treatment. This effect was not manifested in the case of pretreatment of seedling roots with the antioxidant dimethylthiourea (DMTU) and the diamine oxidase inhibitor aminoguanidine. Treatment of seedlings with DMTU and aminoguanidine eliminated the protective effect of putrescine and cadaverine on wheat seedlings under hyperthermia. A conclusion was made about the role of reactive oxygen species, formed due to the action of diamine oxidase, in the realization of the stress-protective action of diamines on wheat seedlings.

Key words: Triticum aestivum, polyamines, putrescine, cadaverine, reactive oxygen species, heat resistance



1. Kokorev A.I., Shvydenko N.V., Yastreb T.O., Kolupaev Yu.E. Induction of heat resistance and antioxidant enzymes of wheat seedlings by exogenous polyamines. Visn. Hark. nac. agrar. univ., Ser. Biol. 2018. 3 (45) : 85-93. (In Russian)
2. Kolupaev Yu.Ye., Karpets Yu.V. Oxidative stress and the state of antioxidative system in wheat coleoptiles at the action of hydrogen peroxide and heating. Visn. Hark. nac. agrar. univ., Ser. Biol. 2 (14) : 42-52. (In Russian)
3. Alcazar R., Altabella T., Marco F., Bortolotti C., Reymond M., Koncz C., Carrasco P., Tiburcio A.F. 2010. Polyamines: molecules with regulatory functions in plant abiotic stress tolerance. Planta. 231 (6) : 1237-1249.
4. Andronis E.A., Moschou P.N., Toumi I., Roubelakis-Angelakis K.A. 2014. Roubelakis-angelakis, peroxisomal polyamine oxidase and NADPH-oxidase cross-talk for ROS homeostasis which affects respiration rate in Arabidopsis thaliana. Front Plant Sci. 5: 132.
5. Aronova E.E., Shevyakova N.I., Stetsenko L.A., Kuznetsov Vl.V. 2005. Cadaverine-induced induction of superoxide dismutase gene expression in Mesembryanthemum crystallinum L. Doklady Biological Sciences. 403 (1-6) : 257-259.
6. Bais H.P., Ravishankar G.A. 2002. Role of polyamines in the ontogeny of plants and their biotechnological applications. Plant Cell, Tissue and Organ Culture. 69 : 1-34.
7. Cavusoglu K., Kabar K. 2007. Comparative effects of some plant growth regulators on the germination of barley and radish seeds under high temperature stress. EurAsian J. BioSciences. 1 (1) : 1-10.
8. Ghosh N., Das S.P., Mandal C., Gupta S., Das K., Dey N., Adak M.K. 2012. Variations of antioxidative responses in two rice cultivars with polyamine treatment under salinity stress. Physiol. Mol. Biol. Plants. 18 (4) : 301-313.
9. Gill S.S., Tuteja N. 2010. Polyamines and abiotic stress tolerance in plants. Plant Signal Behav. 5 (1) : 26-33.
10. Gupta K., Dey A., Gupta B. 2013. Plant polyamines in abiotic stress responses. Acta Physiol. Plant. 35 : 2015-2036.
11. Karpets Yu.V., Kolupaev Yu.E., Vayner A.A. 2015. Functional interaction between nitric oxide and hydrogen peroxide during formation of wheat seedling induced heat resistance. Russ. J. Plant Physiol. 62 (1) : 65-70.
12. Kolupaev Yu.E., Oboznyi A.I, Shvidenko N.V. 2013. Role of hydrogen peroxide in generation of a signal inducing heat tolerance of wheat seedlings. Russ. J Plant Physiol. 60 (2) : 227-234.
13. Kolupaev Yu.E., Kokorev A.I., Shkliarevskyi M.A., Lugovaya A.A,, Karpets Yu.V., Ivanchenko O.E. 2021. Role of NO synthesis modification in the protective effect of putrescine in wheat seedlings subjected to heat stress. Appl. Biochem. Microbiol. 57 (3) : 384-391.
14. Kuznetsov Vl.V., Radyukina N.L., Shevyakova N.I. 2006. Polyamines and stress: Biological role, metabolism, and regulation. Russ. J. Plant Physiol. 53 (5) : 583-604.
15. Pal M., Szalai G. Janda T. 2015. Speculation: Polyamines are important in abiotic stress signaling. Plant Sci. 237 : 16-23.
16. Pang X.M., Zhang Z.Y., Wen X.P., Ban Y., Moriguchi T. 2007. Polyamines, all-purpose players in response to environment stresses in plants. Plant Stress. 1 (2) : 173-188.
17. Sagisaka S. 1976. The occurrence of peroxide in a perennial plant, Populus gelrica. Plant Physiol. 57 (2) : 308-309.
18. Sagor G.H., Berberich T., Takahashi Y., Niitsu M., Kusano T. 2013. The polyamine spermine protects Arabidopsis from heat stress-induced damage by increasing expression of heat shock-related genes. Transgenic Res. 22 (3) : 595-605.
19. Saha J., Brauer E.K., Sengupta A., Popescu S.C., Gupta K., Gupta B. 2015. Polyamines as redox homeostasis regulators during salt stress in plants. Front Environ Sci. 3 : 21.
20. Shevyakova N.I., Rakitin V.Yu., Stetsenko L.A., Aronova E.E., Kuznetsov Vl.V. 2006. Oxidative stress and fluctuations of free and conjugated polyamines in the halophyte Mesembryanthemum crystallinum L. under NaCl salinity. Plant Growth Regul. 50 (1) : 69-78.
21. Szalai G., Pap M., Janda T. 2009. Light-induced frost tolerance differs in winter and spring wheat plants. J. Plant Physiol. 166 (16) : 1826-1831.
22. Wimalasekera R., Villar C., Begum T., Scherer G.F. 2001. Coper amine oxidase1 (CuAO) of Arabidopsis thaliana contributes to abscisic acid- and polyamine-induced nitric oxide biosynthesis and abscisic acid signal transduction. Mol. Plant. 4 : 663-678.