Visn. Hark. nac. agrar. univ., Ser. Biol., 2021, Issue 2 (53), p. 53-60


https://doi.org/10.35550/vbio2021.02.053




ROS-DEPENDENT STRESS-PROTECTIVE EFFECT OF DIAMINES ON WHEAT SEEDLINGS UNDER CONDITIONS OF HYPERTHERMIA


A. I. Kokorev

Dokuchaev Kharkiv National Agrarian University

(Kharkiv, Ukraine)

E-mail: nik20109442@gmail.com


Polyamines are currently considered as stress metabolites of plants that have a direct protective effect on biomacromolecules and are also involved in cellular signal-regulatory processes. The most abundant diamine in plant cells is putrescine. At the same time, another diamine, cadaverine, was found in plants, in particular in cereals, the functions of which have been poorly studied. In this regard, we compared the effects of putrescine and cadaverine on the survival of wheat seedlings (Triticum aestivum L.) after damaging heating. We also studied the effect of these diamines on the content of hydrogen peroxide in roots of seedlings and the probable relationship between the induction of heat resistance of seedlings by diamines and changes in the redox homeostasis of cells. It was found that the pretreatment of seedling roots with both diamines at concentrations of 0.25–2 mM approximately equally increased survival after potentially lethal heat stress (heating in a water thermostat at 45.5°C for 10 min). The most noticeable protective effect of diamines was shown when they were used at a concentration of 1 mM. Under the influence of both putrescine and cadaverine, a transient increase in the content of hydrogen peroxide in roots was observed with a maximum after 2 hours from the start of treatment. This effect was not manifested in the case of pretreatment of seedling roots with the antioxidant dimethylthiourea (DMTU) and the diamine oxidase inhibitor aminoguanidine. Treatment of seedlings with DMTU and aminoguanidine eliminated the protective effect of putrescine and cadaverine on wheat seedlings under hyperthermia. A conclusion was made about the role of reactive oxygen species, formed due to the action of diamine oxidase, in the realization of the stress-protective action of diamines on wheat seedlings.


Key words: Triticum aestivum, polyamines, putrescine, cadaverine, reactive oxygen species, heat resistance

 


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