Visn. Hark. nac. agrar. univ., Ser. Biol., 2021, Issue 2 (53), p. 41-52


https://doi.org/10.35550/vbio2021.02.041




EFFECT OF EPIBRASSINOLIDE AND ITS CONJUGATES WITH SULFURIC ACID ON GROWTH AND SALT RESISTANCE OF HELIANTHUS ANNUUS L.


Y. V. Archybasava1, R. P. Litvinovskaya2

1Pushkin Brest State University

(Brest, Belarus)

E-mail: archibasovayana@gmail.com

2Institute of Bioorganic Chemistry

of the National Academy of Sciences of Belarus

(Minsk, Belarus)


We studied the growth-stimulating effect of conjugates of 24-epibrassinolide with sulfuric acid on the initial growth of Helianthus annuus L. The most pronounced activity relative to the control and epibrassinolide in tests (the effect on the length of the root and hypocotyl of seedlings) is exerted by 2,3-disulfate disodium at a concentration of 10-11 M (148 and 45% relative to the control and 127 and 35 % of epibrassinolide) and 23-monosulfate sodium in a concentration 10-10 M (36 and 25% respectively relative to the control and 15% and 15% of epibrassinolide). In laboratory experiments it was found that under conditions of chloride salinity (150 mM NaCl solution) inhibition of the growth of sunflower seedlings is observed. In plants grown under the influence of salinity (Ks), there is a decrease in the total length of roots by 26% and hypocotyl by 32% relative to plants grown in distilled water (K). The test-seeds under salinization conditions showed suppression of the inhibitory effect of salinization, as well as restoration of growth parameters (increase in the average root length and hypocotyl) when using epibrassinolide at a concentration of 10-9 М by 32% and 58%, 2,3-disulfate disodium at a concentration of 10-10 М by 66 % and 43 %, 3-monosulfate sodium at a concentration of 10-9 М by 31 and 68%, 22-monosulfate sodium in three concentrations by 17-53 and 54-55 % regarding NaCl (150 mM). During the growing season with pre-sowing treatment of seeds with 2,3-disulfate disodium at a concentration of 10-11 M, 23-monosulfate sodium at concentrations of 10-10 M and 10-9 M, suppression of the inhibitory effect of salinization and restoration of growth parameters (an increase in the average root length by 13-33 and hypocotyl by 12-22 %) regarding NaCl (150 mM) were noted. After foliar treatment of plants with 24-epibrassinolide sulfates, a regenerating effect was observed for both root growth by 9-30 and hypocotyl growth by 7-25 % for all studied substances, with the exception of 3-monosulfate sodium. The maximum positive effect on the general antioxidant status of plants was recorded after foliar treatment with 23-monosulfate sodium at concentrations of 10-10 M.


KeywordsHelianthus annuus, brassinosteroids, 24-epibrassinolide, conjugates of epibrassinolide with sulfuric acid, growth, salt stress

 


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