Visn. Hark. nac. agrar. univ., Ser. Biol., 2020, Issue 3 (51), p. 87-97


https://doi.org/10.35550/vbio2020.03.087




POLYMORPHISM AND SELECTION OF FROST-RESISTANT GENOTYPES OF AUTUMN-SOWN BARLEY BY DNA MARKERS OF CHROMOSOME 5H


M. S. Balvinska, O. I. Naguliak, V. I. Fayt

Plant Breeding and Genetics Institute

 – National Center of Seed and Cultivar Investigation

(Odesa, Ukraine)

Е-mail: faytgen@ukr.net


Frost resistance is an important feature in selection of barley (Hordeum vulgare L.). Selection for this characteristic in field conditions, as a result of the absence or insufficient stress load in most years of cultivation, is not always effective. Developments in the field of barley genomics allow to use of molecular markers based on PCR analysis for auxiliary rapid selection of frost-resistant genotypes without stress modeling. The article presents the results of polymorphism study of 28 winter barley and facultative genotypes characterized by frost resistance and hardening speed for a number of loci, mapped in the long arm of chromosome 5H, in the region of key genes for frost resistance Fr-H1 (bin11: WG644, PSR637) and Fr-H2 (bin9-10: Bmag812, OPA17А). Allelic differences were discovered in barley varieties at the OPA17 locus, which are not associated with differences in genotypes in terms of frost resistance. But there is a tendency of the influence differences in the allelic composition of this locus in two groups of varieties – carriers of alternative alleles of this locus – on their differences in the rate of hardening. In the studied samples of barley varieties, DNA polymorphism was not established within the MS-locus Bmag812 and STS-loci WG644, PSR637 of chromosome 5H.


Key words: Hordeum vulgare, barley, frost resistance, PCR markers, 5H chromosome, Fr-H1 and Fr-H2 genes

 


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