Visn. Hark. nac. agrar. univ., Ser. Biol., 2020, Issue 3 (51), p. 37-47


https://doi.org/10.35550/vbio2020.03.037




TISSUE SPECIFICITY OF EXPRESSION OF HEAT SHOCK GENE ATHSP70-10 INARABIDOPSIS THALIANA SEEDLINGS UNDER NORMAL AND STRESS CONDITIONS


L.Ye. Kozeko, E.L. Kordyum

Kholodny Institute of Botany

of National Academy of Science of Ukraine

(Kyiv, Ukraine)

E-mail: liudmyla.kozeko@gmail.com


Mitochondrial heat shock proteins of HSP70 family support protein homeostasis in mitochondria under normal and stress conditions. They provide folding and complex assembly of proteins encoded by mitochondrial genome, as well as import of cytosolic proteins to mitochondria, their folding and protection against aggregation. There are reports about organ-specificity of mitochondrial HSP70 synthesis in plants. However, tissue specificity of their functioning remains incompletely characterized. This problem was studied for mitochondrial AtHSP70-10 in Arabidopsis thaliana seedlings using a transgenic line with uidA signal gene under normal conditions, as well as high temperature and water deficit. Under normal conditions, histochemical GUS-staining revealed the expression of AtHSP70-10 in cotyledon and leaf hydathodes, stipules, central cylinder in root differentiation and mature zones, as well as weak staining in root apex and root-shoot junction zone. RT-PCR analysis of wild-type seedlings exposed to 37°C showed rapid upregulation of AtHSP70-10, which reached the highest level within 2 h. In addition, the gradual development of water deficit for 5 days caused an increase in transcription of this gene, which became more pronounced after 3 days and reached a maximum after 5 days of dehydration. Histochemical analysis showed complete preservation of tissue localization of AtHSP70-10 expression under both abiotic factors. The data obtained indicate the specific functioning of mitochondrial chaperone AtHSP70-10 in certain plant cellular structures.


Key words: Arabidopsis thaliana, HSP70, gene expression, tissue specificity, high temperature, water deficiency

 


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