Visn. Hark. nac. agrar. univ., Ser. Biol., 2020, Issue 3 (51), p. 37-47


L.Ye. Kozeko, E.L. Kordyum

Kholodny Institute of Botany

of National Academy of Science of Ukraine

(Kyiv, Ukraine)


Mitochondrial heat shock proteins of HSP70 family support protein homeostasis in mitochondria under normal and stress conditions. They provide folding and complex assembly of proteins encoded by mitochondrial genome, as well as import of cytosolic proteins to mitochondria, their folding and protection against aggregation. There are reports about organ-specificity of mitochondrial HSP70 synthesis in plants. However, tissue specificity of their functioning remains incompletely characterized. This problem was studied for mitochondrial AtHSP70-10 in Arabidopsis thaliana seedlings using a transgenic line with uidA signal gene under normal conditions, as well as high temperature and water deficit. Under normal conditions, histochemical GUS-staining revealed the expression of AtHSP70-10 in cotyledon and leaf hydathodes, stipules, central cylinder in root differentiation and mature zones, as well as weak staining in root apex and root-shoot junction zone. RT-PCR analysis of wild-type seedlings exposed to 37°C showed rapid upregulation of AtHSP70-10, which reached the highest level within 2 h. In addition, the gradual development of water deficit for 5 days caused an increase in transcription of this gene, which became more pronounced after 3 days and reached a maximum after 5 days of dehydration. Histochemical analysis showed complete preservation of tissue localization of AtHSP70-10 expression under both abiotic factors. The data obtained indicate the specific functioning of mitochondrial chaperone AtHSP70-10 in certain plant cellular structures.

Key words: Arabidopsis thaliana, HSP70, gene expression, tissue specificity, high temperature, water deficiency



1. Kozeko L.Ye. 2018. Involvement of cytosolic heat shock proteins HSP70 and HSP90 in adaptation to water deficit of Arabidopsis thaliana seedlings. Visn. Hark. nac. agrar. univ., Ser. Biol. 2 (44) : 41-49. (In Russian).
2. Nedukha O.M. 2011. Heterophilia in Plants. Kyiv : 192 p. (In Ukrainian).
3. Panchuk I.I., Pyrizhok R.Yu., Volkov R.A. 2008. Expression of Gus signaling gene under control of APX2 promoter in transgenic Arabidopsis plants. In: Genome of plant. Collected scientific articles. Odesa : 108-110. (In Ukrainian).
4. Almoguera C., Coca M.A., Jordano J. 1993. Tissue-specific expression of sunflower heat-shock proteins in response to water-stress. Plant J. 4 : 947-958.
5. Bobrownyzky J. 2006. A method for the imitation of drought stress in Arabidopsis thaliana (L.) Heynh. Інтродукція рослин. 1 : 98-105.
6. Candela H., Martínez-Laborda A., Micol J.L. 1999. Venation pattern formation in Arabidopsis thaliana vegetative leaves. Developmental Biol. 205 : 205-216.
7. Chaudhary R., Baranwal V.K., Kumar R., Sircar D., Chauhan H. 2019. Genome-wide identification and expression analysis of Hsp70, Hsp90, and Hsp100 heat shock protein genes in barley under stress conditions and reproductive development. Funct. Integr. Genomics. 19 : 1007-1022.
8. Dudley P., Wood C.K., Pratt J.R., Moore A.L. 1997. Developmental regulation of the plant mitochondrial matrix located HSP70 chaperone and its role in protein import. FEBS Letters. 417 : 321-324.
9. Fietto L.G., Costa M.D.L., Cruz C.D., Souza A.A., Machado M.A., Fontes E.P.B. 2007. Identification and in silico analysis of the Citrus HSP70 molecular chaperone gene family. Genet. Mol. Biol. 30 (3) suppl. : 881-887.
10. Haralampidis K., Miliony D., Rigas S., Hatzopoulos P. 2002. Combinatorial interaction of cis elements specify the expression of the Arabidopsis AtHsp90-1 gene. Plant Physiol. 129 : 1138-1149.
11. Herrmann J.M., Stuart R.A., Craig E.A., Neupert W. 1994. Mitochondrial heat shock protein 70, a molecular chaperone for proteins encoded by mitochondrial DNA. J. Cell Biol. 127 (4) : 893-902.
12. Kawai A., Nishikawa S., Hirata A., Endo T. 2001. Loss of the mitochondrial Hsp70 functions causes aggregation of mitochondria in yeast cells. J. Cell Sci. 114 : 3565-3574.
13. Lee B., Ahn Y., Kang S.-M., Park Y., Jeon Y.-J., Rho J.M., Kim S.-W. 2015. Stoichiometric expression of mtHsp40 and mtHsp70 modulates mitochondrial morphology and cristae structure via Opa1L cleavage. Molecular Biology of the Cell (MBoC). 26 : 2156-2167.
14. Leng L., Liang Q., Jiang J., Zhang C., Hao Y., Wang X., Su W. 2017. A subclass of HSP70s regulate development and abiotic stress responses in Arabidopsis thaliana. J. Plant Res. 130 : 349-363.
15. Li Q.B., Guy C.L. 2001. Evidence for non-circadian light/dark-regulated expression of Hsp70s in spinach leaves. Plant Physiol. 125 : 1633-1642.
16. Lin B., Wang J., Liu H., Chen R., Meyer Y., Barakat A., Delseny M. 2001. Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana. Cell Stress & Chaperones. 6 (3) : 201-208.<0201:GAOTHS>2.0.CO;2
17. Liu S., Wang J., Cong B., Huang X., Chen K., Zhang P. 2014. Characterization and expression analysis of a mitochondrial heat-shock protein 70 gene from the Antarctic moss Pohlia nutans. Polar Biol. 37 (8) : 1145-1155.
18. Pilot G., Stransky H., Bushey D.F., Pratelli R., Ludewig U., Wingate V.P.M., Frommer W.B. 2004. Overexpression of GLUTAMINE DUMPER1 leads to hypersecretion of glutamine from hydathodes of Arabidopsis leaves. Plant Cell. 16 : 1827-1840.
19. Qi Y., Wang H., Zou Y., Liu C., Liu Y., Wang Y., Zhang W. 2011. Overexpression of mitochondrial heat shock protein 70 suppresses programmed cell death in rice. FEBS Lett. 585 : 231-239.
20. Rizhsky L., Liang H., Shuman J., Shulaev V., Davletova S., Mittler, R. 2004. When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress. Plant Physiol. 134 : 1683-1696.
21. Sarkar N.K., Kundnani P., Grover A. 2013. Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa). Cell Stress Chaperones. 18 (4) : 427-437.
22. Silva-Correia J., Freitas S., Tavares R.M., Lino-Neto T., Azevedo H. 2014. Phenotypic analysis of the Arabidopsis heat stress response during germination and early seedling development. Plant Methods 10 : 7.
23. Su P.H., Li H.M. 2010. Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts. Plant Cell. 22 : 1516-1531.
24. Sung D.Y., Vierling E., Guy C.L. 2001. Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family. Plant Physiol. 126 : 789-800.
25. Swindell W.R., Huebner M., Weber A.P. 2007. Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways. BMC Genomics. 8 : 125.
26. Tang T., Yu A., Li P., Yang H., Liu G., Liu L. 2016. Sequence analysis of the Hsp70 family in moss and evaluation of their functions in abiotic stress responses. Sci. Rep. 6 : 33650.
27. Weigel D., Glazebrook J. 2002. Arabidopsis: A Laboratory Manual. Cold Spring Harbor Laboratory Press: 354 p.
28. Williamson C.L., Dabkowski E.R., Dillmann W.H., Hollander J.M. 2008. Mitochondria protection from hypoxia/reoxygenation injury with mitochondria heat shock protein 70 overexpression. Amer. J. Physiol. Heart Circ. Physiol. 294 : H249-H256.
29. Zhang X.-P., Glaser E. 2002. Interaction of plant mitochondrial and chloroplast signal peptides with the Hsp70 molecular chaperone. Trends Plant Sci. 7 (1) : 14-21.