Visn. Hark. nac. agrar. univ., Ser. Biol., 2020, Issue 2 (50), p. 93-104


https://doi.org/10.35550/vbio2020.02.093



COMBINED EFFECT OF SALICYLIC ACID AND NITRIC OXIDE DONOR ON DEVELOPMENT OF HARDENING-INDUCED FROST RESISTANCE OF WHEAT SEEDLINGS


O. I. Horielova1, M. A. Shkliarevskyi1, N. I. Ryabchun2, L. F. Kabashnikova3, Yu. E. Kolupaev1, 4

1Dokuchaev Kharkiv National Agrarian University
(Kharkiv, Ukraine)

E-mail: plant_biology@ukr.net

2Yurjev Рlant Production Institute
of National Academy of Agrarian Sciences of Ukraine
(Kharkiv, Ukraine)
3Institute of Biophysics and Cell Engineering
of National Academy of Sciences of Belarus
(Minsk, Belarus)
4Karazin Kharkiv National University
(Kharkiv, Ukraine)


It is known that salicylic acid and nitric oxide (NO) are involved in the formation of many adaptive reactions of plants to action of stressors of various natures. There is data that treatment of plants with salicylic acid and nitric oxide donors increases their resistance to low positive temperatures. At the same time, their effect on the resistance of plants to cryostress has been studied deficiently. It is known that some physiological effects of salicylic acid are realized with the participation of NO as a signaling mediator. However, the combined effect of salicylic acid and NO donors on physiological processes responsible for the formation of frost resistance has not been studied yet. We investigate the influence of priming of wheat seed (Triticum aestivum L.) with salicylic acid and NO donor – sodium nitroprusside (SNP) – separately and together on the formation of frost resistance of etiolated wheat seedlings during hardening at 2-4°С. The increase of the seedlings survival after freezing at –6 and –8°С under the influence of salicylic acid and SNP has been shown. The protective effect of the mutual treatment of wheat seeds with salicylic acid (10 μM) and SNP (100 μM) has been more noticeable. Cold hardening of seedlings, as well as the preliminary treatment of seeds with salicylic acid, caused an increase in the activity of superoxide dismutase (SOD), catalase and guaiacol peroxidase, the content of proline and sugars in the seedlings’ tissues. The priming of seed with SNP contributed to an increase in the SOD and catalase activity in wheat seedlings, and in the proline and sugars content. An additional increase in SOD activity and sugars content in seedlings with the combined use of salicylic acid and SNP has been noted. Possible causes of the enhancement of stress-protective effects of salicylic acid and NO donor with their combined action has been discussed.


Key words: Triticum aestivum, salicylic acid, nitric oxide, frost resistance, antioxidant system, osmolytes

 


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