Visn. Hark. nac. agrar. univ., Ser. Biol., 2020, Issue 2 (50), p. 70-82


https://doi.org/10.35550/vbio2020.02.070




FUNCTIONAL STATE OF PLASMALEMMA AND APOPLAST IN BARLEY MESOPHYLL UNDER CONDITIONS OF HYPERTHERMIA AND Bipolaris sorokiniana INFECTION


L. V. Pashkevich, L. F. Kabashnikova, G. E. Savchenko

Institute of Biophysics and Cell Engineering
of National Academy of Sciences of Belarus
(Minsk, Belarus)

E-mail: kabashnikova@mail.ru


The functional state of plasmalemma and apoplast of barley mesophyll was studied under conditions of hyperthermia and infection with fungus Bipolaris sorokiniana (Sacc.) Shoem. As a result of the studies, data were obtained that indicate that under conditions of phytopathogenic infection, in barley mesophyll cells there was an increase of the plasma membrane permeability for free nucleotides, and activation of the K+ ions release from the cells. Short-term heat treatment (40°C, 3 hours) stabilizes ion transport in barley leaves upon infection with the fungus Bipolaris sorokiniana, as evidenced by a decrease in the permeability of cell membranes for a free nucleotides and the release of potassium ions from cells compared with infected leaves. An analysis of pH of the apoplast showed that as a result of the heat shock, the apoplast pH shifts to the alkaline side within a 1 day, while fungal infection causes the pH of apoplast to alkalize only by the 3rd day. And infection on the background of the short-term heat shock already on 2 day led to weak alkalization of the apoplast. Obtained results characterize the effect of short-term hyperthermia on the resistance of barley plants to the fungal pathogen Bipolaris sorokiniana associated with thermally induced changes in the functional characteristics of plasmalemma and apoplast, and indicate the importance of these compartments in formation of responses and adaptation of plants to stress.


Key words: biomembranes, apoplast, apoplast pH, membrane permeability, K+ ions, hyperthermia, Hordeum vulgare, Bipolaris sorokiniana

 


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