Visn. Hark. nac. agrar. univ., Ser. Biol., 2020, Issue 1 (49), p. 62-71


І. V. Kosakivska, L. V. Voytenko, M. M. Shcherbatiuk, V. A. Vasjuk

Kholodny Institute of Botany
of National Academy of Sciences of Ukraine
(Kyiv, Ukraine)

We analyzed the dynamics and distribution of endogenous abscisic (ABA) and indol-3-acetic (IAA) acids in shoots and roots of 14-day-old plants of winter wheat Triticum aestivum L. cv. Podolyanka after short-term hyperthermia (+40°C, 2 h) and the 21-day-old ones during restoration. It was shown that on the early stages of ontogenesis, ABA prevailed in the shoots and IAA in the roots of plants. The accumulation of ABA was more active. The amount of ABA in the shoots of 21-day-old plants increased by 71.4% compared to 14-day-old plants, and by 19.9% in the roots. The accumulation of IAA was less intense. Level of this hormone in the shoots increased by 20.2%, and in the roots – by 1.9%. After heat stress, the ABA content in the shoots and roots of the studied plants increased by 1.3 times and amounted to 39.5 and 19.0 ng/g of fresh weight, respectively. At the same time, the level of IAA in the roots decreased 2.1 times, and in the shoots – 1.7 times and amounted to 51.7 and 44.4 ng/g of fresh weight, respectively. During the recovery period on the 21st day, a further accumulation of ABA was recorded. Thus, the hormone content in the shoots increased by 1.5 times, while in the roots – by 1.9 times and exceeded that of the control plants. An increase in the content of IAA during the recovery period was observed mainly in the shoots (by 80.4%), however, the indices of the studied plants were inferior to the control. In general, the character of accumulation and distribution of ABA and IAA after short-term hyperthermia in wheat plants was different: the amount of ABA increased, while IAA decreased. The interaction of ABA and IAA during hyperthermia and after restoration is discussed.

Key words: Triticum aestivum, abscisic acid, indol-3-acetic acid, hyperthermia, restoration



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