Visn. Hark. nac. agrar. univ., Ser. Biol., 2020, Issue 1 (49), p. 44-53


https://doi.org/10.35550/vbio2020.01.044




POSSIBLE ROLE OF HYDROGEN SULFIDE IN INDUCTION OF ACTIVITY OF ANTIOXIDATIVE ENZYMES AND HEAT RESISTANCE OF WHEAT SEEDLINGS BY PUTRESCINE


А. I. Kokorev1, M. A. Shkliarevskyi1, N. V. Shvydenko1, Yu. E. Kolupaev1, 2

1Dokuchaev Kharkiv National Agrarian University
(Kharkiv, Ukraine)
E-mail:
plant_biology@ukr.net
2Karazin Kharkiv National University
(Kharkiv, Ukraine)


Polyamines in plant cells are considered as stress metabolites that perform not only protective, but also regulatory functions. In particular, they are involved in general regulation of redox homeostasis and activity of antioxidant enzymes. The role of signaling mediators, including hydrogen sulfide, in the implementation of polyamines’ physiological effects remains insufficiently explored. The possible participation of endogenous hydrogen sulfide in the protective effect of diamine putrescine on wheat seedlings (Triticum aestivum L.) under heat stress and activity of antioxidant enzymes in their roots were investigated. Incubation of the roots of intact seedlings in a medium containing 1 mM putrescine caused a transient increase in the hydrogen sulfide generation. The most noticeable effect was observed 2 hours after start of treatment; by 24 hours of incubation, the content of hydrogen sulfide in the roots decreased almost to the control level. Treatment of the roots of seedlings with an inhibitor of the main enzyme for H2S synthesis L-cysteinedesulfhydrase, 0,3 mM potassium pyruvate, partially neutralized the putrescine-induced increase in resistance of the seedlings to damaging heating (10 min at 45°C). A day after heat stress, the content of lipid peroxidation product in the roots increased. This stress effect was attenuated by treatment of the seedlings with putrescine and hydrogen sulfide donor and was enhanced by the inhibitor of H2S synthesis. Under the influence of putrescine, an increase in the activity of superoxide dismutase and catalase in the roots occurred. This effect of polyamine was eliminated by treating seedlings with potassium pyruvate. At the treatment of seedlings with combination of 1 mM putrescine and 0,1 mM hydrogen sulfide donor NaHS an additional increase in the heat resistance of seedlings was noted and also increase in antioxidant enzyme activities. The conclusion is made about the possible participation of hydrogen sulfide as a mediator in the induction by putrescine of the heat resistance of wheat seedlings and their antioxidant system.


Key words: Triticum aestivum, putrescine, hydrogen sulfide, signaling, heat resistance, antioxidant system

 


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