Visn. Hark. nac. agrar. univ., Ser. Biol., 2019, Issue 3 (48), p. 87-94


https://doi.org/10.35550/vbio2019.03.087




ESTIMATION OF EFFICIENCY OF SELECTION OF FROST-RESISTANT PLANTS BY LEAF STOMATAL TRAITS


N. P. Lamari, V. I. Fait

Plant Breeding and Genetic Institute

of National Academy Agrarian Sciences of Ukraine

(Odesa, Ukraine)

E-mail: n.p.lamari@gmail.com


Frost-resistance level (FRL) variation of lines generation F4 – descendants of two alternative plant groups F2 formed by alternative values of stomatal traits: stomata guard cell length (SGCL) and stomata density (SD) was investigated. SGCS and SD were measurement on abaxial third leaf sur-face of seedlings plants in three populations F2: Myronivska 808 × Bezosta 1 (M×B), Odeska 16 × Porada (Od×P), Ulianivka 76 × Odeska chervonokolosa (U×Och). FRL values were identified at stages “09” (“Germination”) and “21-29” (“Tillering”) on scale of the phenological growth stages in lines F4 generation – derived from above given F2 populations. Significant differences were found (t > t0.05) between alternative groups of individual plants F2 for values of stomatal traits in all the above given crossing combinations. Significant increase FRL at “21-29” stage of plants F4 lines in the combinations M×B and U×Och on 16 and 14.9% was associated with a significant decrease in SGCL by 10.2 and 7.6 μm, respectively, and similar increase FRL by 18.7% in crossing Od×P was accompanied by increase in SGCL by 12.8 μm. In crossing combinations M×B and U×Och the FRL values at stage “09” in groups of F4 lines – derived from above given F2 populations with signifi-cantly higher SD (by 14.2 and 7.3 pcs/mm2, respectively) — significantly exceeded (by 15,3 and 19,1%, respectively) of such groups with lower SD. Increase in the FRL at the “21-29” stage of F4 lines Od×P by 18.1% was also accompanied by increase in SD values (by 18.1 pcs/mm2) in F2 plants. It was found that the culling of plants with greater SGCL in the second generation of cross combinations M×B and U×Och will reduce the number of F4 lines by 50 and 51%, and also increase the average FRL at stages “09” and “21-29” of F4 lines by 7,9 and 6.9%, respectively. In turn, the selection of plants with the highest SD in the F2 generation of these combinations will reduce the number of F4 lines by 52 and 47%, and also increase the average FRL at stages "09" and "21-29" by 9 and 2.6%, respectively. In the crossing combination Od×P, the culling of plants with lower SGCL and higher SD, whose share of the total number of F2 plants was 52 and 38%, respectively, will make it possible to increase the average FRL at the “21-29” stage of F4 lines by 7.6 and 18.2%.


Key words: heavy metals, auxins, gibberellins, cytokinins, abscisic acid, salicylic acid, ethylene, jasmonates, brassinosteroids, reactive oxygen species, resistance to stress

 


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