Visn. Hark. nac. agrar. univ., Ser. Biol., 2019, Issue 3 (48), p. 75-86


https://doi.org/10.35550/vbio2019.03.075




INDUCTION OF NONSPECIFIC RESISTANCE OF SEEDLINGS OF SCOTCH PINE BY INFLUENCE OF 24-EPIBRASSINOLIDE


M. A. Shkliarevskyi, D. A. Taraban, Yu. P. Pavlov, Yu. V. Karpets

Dokuchaev Kharkiv National Agrarian University

(Kharkiv, Ukraine)


Young seedlings of Scotch pine (Pinus sylvestris L.) in the nurseries often undergo the affections by complex of pathogenic fungi, causing the coniferous root rot. At the same time the pine at the early stages of development is rather responsive to the drought and to others abiotic stressors. Based on this, the search of methods of increase in nonspecific resistance of coniferous plants is urgent. Brassinosteroids find broad application in the crop production for the increase in growth and resistance of plants to abiotic and biotic factors. However their influence on stress-resistance of woody plants and, especially, coniferous is almost not investigated. The influence of foliar treatment of pine seedlings with the 24-epibrassinolide (24-EBL) on their growth and resistance to the coniferous root rot and drought have been studied. At normal watering four-time spraying of plants by 24-EBL in concentration of 50 and 200 nM increased their growth in height. Also under the influence of brassinosteroid the increase in content of chlorophyll and carotinoids in the above-ground part of plants was registered. The treatment of plants with 24-EBL considerably reduced their affection by the coniferous root rot. At the action of soil drought (the decrease of humidity of substrate up to 25-30% of full moisture capacity), the treatment of plants with 24-EBL reduced the suppression of biomass accumulation, promoted the preservation of water content, close to control variant, and considerably reduced the water deficiency. Foliar treatment of pine seedlings with 24-EBL can be considered as the effective method for the increase in resistance of coniferous plants to the stressors of various nature.


Key words: Pinus sylvestris, brassinosteroids, 24-epibrassinolide, growth, coniferous root rot, drought resistance

 


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