Visn. Hark. nac. agrar. univ., Ser. Biol., 2019, Issue 3 (48), p. 75-86


M. A. Shkliarevskyi, D. A. Taraban, Yu. P. Pavlov, Yu. V. Karpets

Dokuchaev Kharkiv National Agrarian University

(Kharkiv, Ukraine)

Young seedlings of Scotch pine (Pinus sylvestris L.) in the nurseries often undergo the affections by complex of pathogenic fungi, causing the coniferous root rot. At the same time the pine at the early stages of development is rather responsive to the drought and to others abiotic stressors. Based on this, the search of methods of increase in nonspecific resistance of coniferous plants is urgent. Brassinosteroids find broad application in the crop production for the increase in growth and resistance of plants to abiotic and biotic factors. However their influence on stress-resistance of woody plants and, especially, coniferous is almost not investigated. The influence of foliar treatment of pine seedlings with the 24-epibrassinolide (24-EBL) on their growth and resistance to the coniferous root rot and drought have been studied. At normal watering four-time spraying of plants by 24-EBL in concentration of 50 and 200 nM increased their growth in height. Also under the influence of brassinosteroid the increase in content of chlorophyll and carotinoids in the above-ground part of plants was registered. The treatment of plants with 24-EBL considerably reduced their affection by the coniferous root rot. At the action of soil drought (the decrease of humidity of substrate up to 25-30% of full moisture capacity), the treatment of plants with 24-EBL reduced the suppression of biomass accumulation, promoted the preservation of water content, close to control variant, and considerably reduced the water deficiency. Foliar treatment of pine seedlings with 24-EBL can be considered as the effective method for the increase in resistance of coniferous plants to the stressors of various nature.

Key words: Pinus sylvestris, brassinosteroids, 24-epibrassinolide, growth, coniferous root rot, drought resistance



1. Avalbaev А.M., Yuldashev R.A., Shakirova F.M. 2006. Physiological effects of phytohormones brassinosteroids on plants. Uspekhi Sovrem. Biologii. 126 (2) : 192-200.
2. Grodnitskaya I.D., Kuznetsova G.V. 2012. Diseases of Pinus sylvestris L. and Pinus sibirica Du Tour in geographical cultures and forest nurseries of the Krasnoyarsk Territory and Khakassia. Khvoynyye Boreal’noy Zony. 27 (3-4) : 55-60.
3. Karpets Y.V., Kolupaev Y.E. 2018. Participation of nitric oxide in 24-epibrassinolide-induced heat resistance of wheat coleoptiles: functional interactions of nitric oxide with reactive oxygen species and Ca ions. Russ. J. Plant Physiol. 65 (2) : 177-185.
4. Karpets Yu.V., Shklyarevskiy M.A., Lugova G.A. 2018a. Induction of nonspecific resistance of scotch pine seedlings under influence of no donor sodium nitroprusside. 1. Increase of resistance against root rot disease . Visn. Hark. nac. agrar. univ., Ser. Biol. 1 (43) : 57-65.
5. Karpets Yu.V., Shklyarevskiy M.A., Lugova G.A. 2018b. Induction of nonspecific resistance of scotch pine seedlings under influence of no donor sodium nitroprusside. 2. Increase in resistance against soil drought. Visn. Hark. nac. agrar. univ., Ser. Biol. 1 (43) : 66-75.
6. Karpets Yu.V., Vayner A.A., Oboznyi O.I., Yastreb T.O. 2014. Induction of resistance of seedlings of scotch pine to infectious damping-off (fusarial wilt) by influence of exogenous salicylic acid. Visn. Hark. nac. agrar. univ., Ser. Biol. 2 (32) : 63-69.
7. Kolupaev Yu.E., Vayner A.A. 2014. Mechanisms of the stress-protective effect of brassinosteroids on plants. Agrochimiya. 7 : 69-84.
8. Kolupaev Yu.E., Karpets Yu.V., Yastreb T.O., Lugova G.A. 2016. Signal mediators in realization of physiological effects of stress phytohormones. Visn. Hark. nac. agrar. univ., Ser. Biol. 1 (37) : 42-62.
9. Kuzmichev E.P., Sokolova E.S., Mozolevskaya E.G. 2004. Woody Plant Diseases: A Guide. Moscow : 120 p.
10. Manaenkov A.S. 2009. Features of the water regime of the root layer and drought tolerance of pine crops. Lesovedenie. 2 : 52-61.
11. Pustovoitova T.N., Zhdanova N.E., Zholkevich V.N. 2001. Increasing drought tolerance of plants under the influence of epibrassinolide. Proc. Acad. Sci. 376 (5) : 697-700.
12. Sokolova E.S., Galasyeva T.V. 2005. Mushroom diseases of conifers in nurseries and young growth. Moscow : 43 p.
13. Shakirova F.M. 2001. Nonspecific resistance of plants to stress factors and its regulation. Ufa : 160 p.
14. Shlyk A.A. 1971. Determination of chlorophylls and carotenoids in green leaf extracts. In: Biochemical methods in plant physiology. Ed. Pavlinova O.A. Moscow : 154-170.
15. Yuldashev R.A. 2009. Regulation of metabolism of cytokinins by 24-epibrassinolide in wheat plants: Thesis diss. ... cand. biol. sci. Ufa: 23 p.
16. Bajguz A., Hayat S. 2009. Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiol. Biochem. 47 : 1-8.
17. Bartoli C.G., Gomez F., Martinez D.E., Guiamet J.J. 2004. Mitochondria are the main target for oxidative damage in leaves of wheat (Triticum aestivum L.). J. Exp. Bot. 55 : 1663-1669.
18. Cheon, J., Park, S.Y., Schulz, B., and Choe, S. 2010. Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction. BMC Plant Biol. 10 : 270.
19. Clouse S.D. Brassinosteroids. In: The Arabidopsis Book. 2011 : e0151.
20. Cuttriss A.J., Pogson B.J. 2004. Carotenoids. Plant Pigments and Their Manipulation, editor Davies K.M. Boca Raton : 57-91.
21. Du J., Gerttula S., Li Z., Zhao S.T., Liu Y.L., Liu Y., Lu M.Z., Groover A.T. 2019. Brassinosteroid regulation of wood formation in poplar. New Phytol.
22. Foyer C.H., Shigeoka S. 2011. Understanding oxidative stress and antioxidant functions to enhance photosynthesis. Plant Physiol. 155 : 93-100.
23. Cukor, J., Rasáková, N. M., Linda, R., Linhart, L., Gutsch, M. R. and Kunes, 2018. Effects of BrassinosteroidApplication on Seed Germination of Scots Pine under Standard and Heat Stress Conditions. Baltic Forestry 24 (1): 60-67.
24. Howell W.M., Keller G.E., Kirkpatrick J.D., Jenkins R.L., Hunsinger R.N., McLaughlin E.W. 2007. Effects of the plant steroidal hormone, 24-epibrassinolide, on the mitotic index and growth of onion (Allium cepa) root tips. Genet. Mol. Res. 6 : 50-58.
25. Li Y.H., Liu Y.J., Xu X.L., Jin M., An L.Z., Zhang H. 2012. Effect of 24-epibrassinolide on drought stress-induced changes in Chorispora bungeana. Biol. Plant. 56 : 192-196.
26. Nakashita H., Yasuda M., Nitta T., Asami T., Fujioka S., Arai Yu., Sekimata. K., Takatsuto S., Yamaguchi I., Yoshida S. 2003. Brassinosteroid functions in a broad range of disease resistance in tobacco and rice. Plant J. 33 : 887-898.
27. Nogues S., Baker N.R. 2000. Effects of drouht on photosynthesis in Mediterranean plants grown under UV-B radiation. J. Exp. Bot. 51 : 1309-1317.
28. Sadeghi F., Shekafandeh A. 2014. Effect of 24-epibrassinolide on salinity-induced changes in loquat (Eriobotrya japonica Lindl). Journal of Applied Botany and Food Quality 87 : 182-189.
29. Santos C.V. 2004. Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves. Sci. Horticult. 103 : 93-99.
30. Xia X.J., Wang Y.J., Zhou Y.H., Tao Y., Mao W.H., Shi K., Asami T., Chen Z., Yu J.Q. 2009. Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber. Plant Physiol. 150 : 801-814.
31. Xia X.J., Zhou Y.H., Ding J., Shi K., Asami T., Chen Z., Yu J.Q. 2011. Induction of systemic stress tolerance by brassinosteroid in Cucumis sativus. New Phytol. 191 : 706-720.
32. Yuan G.F., Jia C.G., Li Z., Sun B., Zhang L.P., Liu N., Wang Q.M. 2010. Effect of brassinosteroids on drought resistance and abscisic acid concentration in tomato under water stress. Sci. Horticult. 126 : 103-108.