Visn. Hark. nac. agrar. univ., Ser. Biol., 2019, Issue 2 (47), pp. 92-100


S. V. Mishchenko

Institute of Bast Crops of National Academy of Agrarian Science of Ukraine

(Hlukhiv, Sumy region, Ukraine)

Linum usitatissimum L. convar. elongatum (Hlinum variety) is mostly capable to form the callus on the hypocotyl segments provided that it is cultivated on a Murashige and Skoog medium supplemented with 30 g/L sucrose, a 16 h photoperiod, a 60–80% relative humidity, an 22-24°C air temperature, and under the influence of only auxins (i), only cytokinins (ii) and combinations of auxins and cytokinins (iii) of exogenous origin. The highest frequency of organogenesis was in the variant with 1,0 mg/L 6-benzylaminopurine (BAP), 0.3 mg/L kinetine (cytokinins only), a combination of 1-naphthylacetic acid (NAA) and BAP, indole-3-acetic acid (IAA) and BAP. Studies with a large variation range of BAP concentrations indicate that in the presence of auxin NAA (0.05 mg/L in all variants) the efficiency of callus formation and shoots regeneration was higher. However, the use of only BAP can be quite a distinct technique for the induction of callus and organogenesis. For the studied species in general and this genotype in particular the synthesis of auxins of endogenous origin in the presence of cytokinins of exogenous origin is sufficient for organogenesis. Increasing of the concentration of BAP up to 3.0 mg/L inhibited the callus formation and shoots regeneration. The optimal concentrations of phytohormones can be expressed by inequality 1.0 ≤ BAP ≤ 1.75, and when 0.05 mg/L of NAA is added to the medium, inequality 0.5 ≤ BAP ≤ 2.0. The greatest efficiency of callus formation and organogenesis was found in variants 1.5 mg/L BAP (i), 1.25 mg/L BAP and 0.05 mg/L NAA (ii). Reaction of L. usitatissimum L. convar. elongatum on the effect of phytohormones compared to L. usitatissimum L. convar. mediterraneum was different.

Key words: Linum usitatissimum, in vitro, phytohormones, 6-benzylaminopurine, callus, organogenesis



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