Visn. Hark. nac. agrar. univ., Ser. Biol., 2019, Issue 2 (47), pp. 71-81


https://doi.org/10.35550/vbio2019.02.071




COMBINED INFLUENCE OF SODIUM NITROPRUSSIDE AND 24-EPIBRASSINOLIDE ON REDOX-HOMEOSTASIS AND HEAT RESISTANCE OF WHEAT PLANTLETS


M. A. Shkliarevskyi, Yu. V. Karpets, G. A. Lugova, O. I. Horielova

Dokuchaev Kharkiv National Agrarian University
(Kharkiv, Ukraine)


Brassinosteroids are one of the key classes of phytohormones, involved into the adaptation of plants to the action of adverse factors. Their effects are realized with the participation of signal mediators, including reactive oxygen and nitrogen species. Nitric oxide (NO) is capable to induce various adaptive responses of plants. At the same time physiological effects of combined influence of NO donors and exogenous brassinosteroids on plants are almost not investigated. The separate and combined influences of treatment of seeds or roots of plantlets of wheat (Triticum aestivum L.) with NO donor sodium nitroprusside (SNP) and 24-epibrassinolide (24-EBL) in the wide range of concentration on their heat resistance and indicators the redox homeostasis have been studied. SNP in the concentration range of 0,2-2 mM caused the increase in resistance of plantlets to the damaging heating. The treatment of plantlets or seeds with 24-EBL in concentration of 20-200 nM had the same effect. The cotreatment of plantlets or seeds with two compounds in low concentration (0,2 mM of SNP and 20 nM of 24-EBL) had the greatest protective effect, which exceeded the influence of the optimal concentrations of each of the compounds separately. The treatment of plantlets with SNP and 24-EBL or their combination in optimum concentration caused the transitional increase in the content of hydrogen peroxide (H2O2) in roots. At the same time high concentration of these compounds and especially their combination caused longer effect of growth of quantity of H2O2 in roots. The treatment of seeds with 24-EBL in high concentration and its combination with SNP also affected the increase of content of hydrogen peroxide in roots. SNP in moderate concentration and its combination with 24-EBL reduced to some extent the content of malonic dialdehyde in roots, at the same time their high concentration and especially their combination with 24-EBL caused the manifestation of the effect of oxidative stress. It is noted that the effect of synergism of stressprotective influence of SNP and 24-EBL at their use in rather low concentration can be bound to the intensifying of the signal transduction of brassinosteroids to the genetic apparatus at the additional influx of NO, which is one of the mediators in realization of physiological action 24-EBL, into the cells. At the same time the cotreatment with SNP and 24-EBL in high concentration led to the accumulation of hydrogen peroxide and products of lipid peroxidation.


Key words: Triticum aestivum, nitric oxide (NO), 24-epibrassinolide, heat resistance, redox homeostasis, hydrogen peroxide, malonic dialdehyde

 


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