Visn. Hark. nac. agrar. univ., Ser. Biol., 2018, Issue 1 (43), с. 66-75


Yu. V. Karpets, M. A. Shklyarevskiy, G. A. Lugova

Dokuchaev Kharkiv National Agrarian University
(Kharkiv, Ukraine)

The influence of nitric oxide donor sodium nitroprusside (SNP) on the resistance of seedlings of Scotch pine (Pinus silvestris L.) against the soil drought (declining of humidity of substrate up to 25-30% of full moisture capacity) have been investigated. Spraying of plants with SNP in concentration of 0,2 and 0,5 mM caused small intensifying of linear growth of plants and accumulation of biomass at optimum watering. Under the drought condition the positive influence of SNP in concentration range of 0,2-2,0 mM on the growth of plants in height and accumulation of fresh and dry biomass was more essential. After the influence of 10-day drought the water deficiency of control plants amounted about 20%, their treatment with SNP reduced this indicator to 10-12%. The drought caused lowering of total maintenance of chlorophyll and carotinoids and also reduction of ratio of chlorophyll a/b, treatment of plants with the donor of nitric oxide promoted preservation of pool of photosynthetic pigments close to quantity in variant with normal moistening. Also the plants treated with SNP in the drought condition had no lowering of content of anthocyans and flavonoids, absorbing in the wave range of UF-B, which was observed in control plants. The conclusion about the role of the signalling molecule NO in the induction of adaptive responses of Scotch pine against the influence of abiotic stressor – soil drought – is made.

Key words: Pinus silvestris, drought, nitric oxide, sodium nitroprusside, resistance, photosynthetic pigments, anthocyans, flavoniods



1. Verzunov A.I., Mehedova L.G. 2007. The rate of water loss by pine shoots from geographical crops during artificial dehydration. Lesovedenie. 3 : 66-70.
2. Zhuk I.V., Musiienko M.M. 2010. The influence of nitric oxide on wheat plants in the drought conditions. Bulletin of Agricultural Science. 5 : 32-34.
3. Karpets Yu.V. 2016. Influence of no donor on content of pigments in leaves, growth and productivity of spring wheat (Triticum aestivum L.). Visn. Hark. nac. agrar. univ., Ser. Biol. 3(39) 48-56.
4. Karpets Yu.V., Kolupaev Yu.E., Grigorenko D.O., Firsova K.M. 2016. Response of barley plants of various genotypes to soil drought and influence of nitric oxide donor. Visn. Hark. nac. agrar. univ., Ser. Biol. 3(38) : 84-105.
5. Karpets Yu.V., Kolupaev Yu.E., Yastreb T.O. 2011.Effect of sodium nitroprusside on heat resistance of wheat coleoptiles: Dependence on the formation and scavenging of reactive oxygen species. Russ. J. Plant Physiol. 58(6) : 1027-1033.
6. Karpets Yu.V., Kolupaev Yu.E., Shvydenko M.V., Yastreb T.O. 2015. Influence of sodium nitroprusside on pigmental complex of leaves and productivity of millet in adverse conditions. Visn. Hark. nac. agrar. univ., Ser. Biol. 3(36) : 38-44.
7. Karpets Yu.V., Shklyarevskiy M.A., Lugova G.A. 2018. Induction of nonspecific resistance of scotch pine seedlings under influence of no donor sodium nitroprusside. 1. Increase of resistance against root rot disease . Visn. Hark. nac. agrar. univ., Ser. Biol. 1(43) : 57-65.
8. Kolupaev Y.E., Yastreb T.O. 2015. Physiological functions of nonenzymatic antioxidants in plants. Hark. nac. agrar. univ., Ser. Biol. 2(35) : 6-25.
9. Kuznetsova N.F. 2010. Sensitivity of scotch pine generative sphere to drought. Lesovedenie. 6 : 46-53.
10. Manaenkov A.S. 2009. Features of the water regime of the root layer and drought tolerance of pine crops. Lesovedenie. 2 : 52-61.
11. Tian X.R., Lei Y.B. 2007. Physiological responses of wheat seedlings to drought and UV-B radiation. Effect of exogenous sodium nitroprusside application. Russ. J. Plant Physiol. 54(5) : 676-682.
12. Shlyk A.A. 1971. Determination of chlorophylls and carotenoids in green leaf extracts. In: Biochemical methods in plant physiology. Ed. Pavlinova O.A. Moscow : 154-170.
13. Cuttriss A.J., Pogson B.J. 2004. Carotenoids. In: Plant Pigments and Their Manipulation. Ed. Davies K.M.. Boca Raton : 57-91.
14. Floryszak-Wieczorek J., Milczarek G., Arasimowicz M., Ciszewski A. 2006. Do nitric oxide donors mimic endogenous NO-related response in plants? Planta. 224 : 1363-1372.
15. Galatro A., Puntarulo S. 2014. An update to the understanding of nitric oxide metabolism in plants In: Nitric Oxide in Plants: Metabolism and Role in Stress Physiology. Springer International Publishing : 3-15.
16. Gill S.S., Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48 : 909-930.
17. Khan M.N., Mobin M., Mohammad F., Corpas F.J. (eds.). 2014. Nitric oxide in plants: metabolism and role in stress physiology. Heidelberg : Springer : 302 p.
18. Khlestkina E.K. 2013. The adaptive role of flavonoids: emphasis on cereals. Cereal Res. Commun. 41 : 185-198.
19. Krasylenko Y.A., Yemets A.I.,, Sheremet Y.A., Blume B. 2012. Nitric oxide as a critical factor for perception of UV-B irradiation by microtubules in Arabidopsis. Physiol. Plant. 145 : 505-515.
20. Lu D., Zhang X., Jiang J., An G.Y., Zhang L.R., Song P. 2005. NO may function in the downstream of H2O2 in ABA-induced stomatal closure in Vicia faba L. J. Plant Physiol. Mol. Biol. 31 : 62-70.
21. Neill S.O., Gould K.S. 2003. Anthocyanins in leaves: light attenuators or antioxidants? Functional Plant Biol. 30(8) : 865-873.
22. Nogues S., Baker N.R. 2000. Effects of drouht on photosynthesis in Mediterranean plants grown under UV-B radiation. J. Exp. Bot. 51 : 1309-1317.
23. Pietrini F., Massacci A. 1998. Leaf anthocyanin content changes in Zea mays L. grown at low temperature: Significance for the relationship between the quantum yield of PS II and the apparent quantum yield of CO2 Photosynthesis Res. 58 : 213-219.
24. Ruan H.H., Shen W.B., Xu L.L. 2004. Nitric oxide involved in the abscisic acid induced proline accumulation in wheat seedling leaves under salt stress. Acta Bot. Sinica. 46 : 1307-1315.
25. Singh P., Shah K. 2017. An update on effects of nitric oxide under abiotic stresses in higher plants. Adv. Plant Physiol. 15 : 283-306.
26. Tan J., Zhao H., Hong J., Han Y., Li H., Zhao W. 2008. Effects of exogenous nitric oxide on photosynthesis, antioxidant capacity and proline accumulation in wheat seedlings subjected to osmotic stress. World J. Agricult. Sci. 4 : 307-313.
27. Wang S.H., Zhou Z.Y., He Q.Y., Xiaopeng W., Song L , Lu X. 2007. Nitric oxide alleviates the nickel toxicity in wheat seedlings. Acta Bot. Yunnanica. 29(1) : 115-121.
28. Wang H., Zhang H., Jiang S.J., Zhang L., He Q.Y., He H.Q. 2010. Effects of the nitric oxide donor sodium nitroprusside on antioxidant enzymes in wheat seedling roots under nickel stress. Russ. J. Plant Physiol. 57 : 833-839.
29. Wu X., Zhu W., Zhang H., Ding H., Zhang H.J. 2011. Exogenous nitric oxide protects against salt-induced oxidative stress in the leaves from two genotypes of tomato (Lycopersicom esculentum Mill.). Acta Physiol. Plant. 33 : 1199-1209.
30. Zhang Y., Wang L., Liu Y., Zhang Q., Wei Q., Zhang W. 2006. Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast. Planta. 224 : 545-555.