Visn. Hark. nac. agrar. univ., Ser. Biol., 2018, Issue 1 (43), с. 66-75


https://doi.org/10.35550/vbio2018.01.066




INDUCTION OF NONSPECIFIC RESISTANCE OF SCOTCH PINE SEEDLINGS UNDER INFLUENCE OF NO DONOR SODIUM NITROPRUSSIDE.
2. INCREASE IN RESISTANCE AGAINST SOIL DROUGHT


Yu. V. Karpets, M. A. Shklyarevskiy, G. A. Lugova

Dokuchaev Kharkiv National Agrarian University
(Kharkiv, Ukraine)
E-mail:
plant.biology.knau@gmail.com


The influence of nitric oxide donor sodium nitroprusside (SNP) on the resistance of seedlings of Scotch pine (Pinus silvestris L.) against the soil drought (declining of humidity of substrate up to 25-30% of full moisture capacity) have been investigated. Spraying of plants with SNP in concentration of 0,2 and 0,5 mM caused small intensifying of linear growth of plants and accumulation of biomass at optimum watering. Under the drought condition the positive influence of SNP in concentration range of 0,2-2,0 mM on the growth of plants in height and accumulation of fresh and dry biomass was more essential. After the influence of 10-day drought the water deficiency of control plants amounted about 20%, their treatment with SNP reduced this indicator to 10-12%. The drought caused lowering of total maintenance of chlorophyll and carotinoids and also reduction of ratio of chlorophyll a/b, treatment of plants with the donor of nitric oxide promoted preservation of pool of photosynthetic pigments close to quantity in variant with normal moistening. Also the plants treated with SNP in the drought condition had no lowering of content of anthocyans and flavonoids, absorbing in the wave range of UF-B, which was observed in control plants. The conclusion about the role of the signalling molecule NO in the induction of adaptive responses of Scotch pine against the influence of abiotic stressor – soil drought – is made.


Key words: Pinus silvestris, drought, nitric oxide, sodium nitroprusside, resistance, photosynthetic pigments, anthocyans, flavoniods

 


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