Visn. Hark. nac. agrar. univ., Ser. Biol., 2018, Issue 1 (43), с. 46-56


https://doi.org/10.35550/vbio2018.01.046




INFLUENCE OF EXOMETABOLITES OF FUSARIUM MICROMYCETES ON GROWTH REACTION AND PEROXIDASE ACTIVITY IN WHEAT LINES ISOGENIC BY VRN GENES IN VIVO AND IN VITRO


O. O. Avksentiieva1, 2, N. V. Terentiieva1

1Karazin Kharkiv National University
(Kharkiv, Ukraine)
E-mail:
avksentyeva@karazin.ua
2Taras Shevchenko National University of Kyiv
(Kyiv, Ukraine)


On models of monogenodominant, isogenic by VRN genes, lines of soft wheat genotypes of Myronivskaya 808, level of their response to the action of exometabolites (culture filtrate, CF) of species Fusarium oxysporum and Fusarium moniliforme in the in vivo and in vitro system was studied. In growth experiments (in vivo) the phytotoxic action of CF on leaves of plants, their influence on growth processes and activity of peroxidase were determined. In vitro culture there were determined growth of the callus with growth index (GI), its morphological and cytological characteristics, and activity of peroxidase. It has been established that fast-growing VRN-A1a and VRN-D1a lines exhibit a higher response rate to the influence of CF strains of both species of Fusarium than the VRN-B1a line and the slow-growing variety, this was shown on all indicators, both in vivo and in vitro. Moreover, the level of manifestation of this reaction in all the lines to the action of CF in vitro was similar to that was manifested in vivo. The obtained results suggest that the VRN genes indirectly contribute to the formation of resistance to exоmetabolites g. Fusarium, and in vitro culture is an adequate system for investigating the effects of specific genes (in particular, VRN) on course of stress reactions in wheat plants.


Key words: Triticum aestivum, Fusarium oxysporum, Fusarium moniliforme, VRN genes, NILs, callus culture, phytotoxicity, growth index, peroxidase, resistance to phytopathogens

 


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