Visn. Hark. nac. agrar. univ., Ser. Biol., 2018, Issue 1 (43), с. 40-45


L. M. Babenko

Kholodny Institute of Botany
of National Academy of Sciences of Ukraine
(Kyiv, Ukraine)

Effects of short-term thermal (+40°C, 2 h) and cold (+4°C, 2 h) stresses on lipoxygenase (LОX) activity in Triticum spelta L. were investigated. For the first time in above-ground part of T. spelta there were identified three molecular forms of 9-LOX namely LOX-1 (рНopt. 5,5), LOX-2 (рНopt. 5,8) and LOX-3 (рНopt. 6,2), and there in roots one 9-LOX (рНopt. 6,0). Activity of LOX-1 and LOX-2 in above-ground part and LOX in roots was found to increase significantly following a short-term hyperthermia. Intensity of response to a heat stress considerably exceeded that to a cold effect. It is assumed that various molecular forms of LOX that appear to have different cell localization are differentially involved in adaptation of T. spelta plants to a temperature stress.

Key words: Triticum spelta, lipoxygenase, hypothermia, hyperthermia



1. Golik O.V., Tverdokhleb E.V., Pozdnyakov V.V., Didenko S.Yu., Boguslavskiy R.L. 2016. Types of wheat for organic agriculture. In: Basic and applied research in bioorganic agriculture in Russia, the CIS and the EU: International scientific and practical conference. Materials of reports and messages, vol. 1. Moscow : 368-378.
2. Gospodarenko G.M.., Kostogryz P.V., Lyubich V.V., Pariy M.F., Poltoretskiy I.O., Polyanetska I.O., Ryabovol L.O., Ryabovol Ya.S., Sukhodum O.G. 2016. Spelta wheat. Kyiv : 300 p.
3. Kosakisvska I.V., Babenko L.M., Vasyuk V.A., Voytenko L.V. 2017. Hyperthermia and ground drought effects on growth, content of photosynthetic pigments and epidermis microstructure in leaf of Triticum spelta L. Visn. Hark. nac. agrar. univ., Ser. Biol. 3(42) : 81-91.
4. Kosakivska I.V., Babenko L.M., Ustinova A.Yu., Skaterna T.D., Demirevska K. 2012. The influence of temperature conditions on lipoxygenase activity in seedling of rape Brassica napus var. Oleifera. Reports of the National Academy of Sciences of Ukraine. 6 : 134-137.
5. Morgun V.V., Sichkar S.M., Pochinok V.M., Golik O.V., Chugunkova T.V. 2015. Analysis of productivity structure of collection samples of rare wheat species. Factors in experimental evolution of organisms. 16 : 136-140.
6. Pokotylo I.V., Kolesnikov Y.S., Derevyanchuk M.V., Kharitonenko A.I., Kravets V.S. 2015. Lipoxygenases and plant cell metabolism regulation. Ukr. Biochem. J. 87(2) : 41-55.
7. Andreou A, Feussner I. 2009. Lipoxygenases - Structure and reaction mechanism Phytochem. 70 : 1504-1510.
8. Babenko L.M., Kosakivska I.V., Akimov Yu.A., Klymchuk D.O., Skaternya T.D. 2014. Effect of temperature stresses on pigment content, lipoxygenase activity and cell ultrastructure of winter wheat seedlings. Genet. Plant Physiol. 4(1-2) : 117-125
9. Babenko L.M., Kosakivska І.V., Skaterna T.D. 2015. Jasmonic acid: a role in the regulation of biotechnology and biochemical processes in plants. Biotechnol. Acta. 82(2) : 36-51.
10. Babenko L.M., Shcherbatiuk M.M., Skaterna T.D, Kosakivska I.V. 2017.Lipoxygenases and their metabolites in formation of plant stress tolerance. Ukr. Biochem.J. 89(1) : 5-21.
11. Babenko L.M. 2017. Effect of temperature on lipoxygenase activity in varieties of Triticum aestivum L. differing in resistance to abiotic stressors. J. Stress Physiol. Biochem. 13(4) : 95-103.
12. Barlow K.M., Christy B.P., O'Leary G.J., Riffkin P.A., Nuttall J.G. 2015. Simulating the impact of extreme heat and frost events on wheat crop production: a review. Field Crops Res. 17 : 109-119.
13. Borrego E.J., Kolomiets M.V. 2012. Lipid-mediated signaling between fungi and plants. In: Biocommunication of Fungi. New York: Springer : 249-260.
14. Borrego E.J., Kolomiets M.V. 2016. Synthesis and functions of jasmonates in maize. Plants. 5(4). : 41-69.
15. Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein uti-lizing the principle of protein-dye binding. Anal. Biochem. 72 : 248-254.
16. Braidot E., Petrussa E., Micolini S. 2004. Biochemical and immunochemical evidences for the presence of lipoxygenase in plant mitochondria. J. Exp. Bot. 55 : 1655-1662.
17. Christensen S.A., Kolomiets M.V. 2011. The lipid language of plant-fungal interactions. Fungal Genet. Biol. 48 : 4-14.
18. Feussner I., Wasternack C. 2002. The lipoxygenase pathway. Annu. Rev. Plant Biol. 53 : 275-297.
19. Gibian M.J., Vandenberg P. 1987. Product yield in oxygena-tion of linoleate by soybean lipoxygenase: The value of the molar extinction coefficient in the spectrophotometric . Anal. Biochem. 163 : 343-349.
20. Hatfield J., Prueger J. 2015. Temperature extremes: Effect on plant growth and development. Weather and Climate Extremes. 10 : 4-10.
21. Hurkman W.J., Wood D.F. 2011.High temperature during grain fill alters the morphology of protein and starch deposits in the starchy endosperm cells of develop-ing wheat (Triticum aestivum L.) grain. J. Agric. Food Chem. 59 :4938-4946.
22. Ivanov I., Heydeck D., Hofheinz K., Roffeis J., O'Donnell V.B., Kuhn H., Walther M. 2010. Molecular enzymology of lipoxygenases. Arch. Biochem. Biophys. 503 : 161-174.
23. Joo Y.C., Oh D.K. 2012. Lipoxygenases: Potential starting bi-ocatalysts for the synthesis of signaling compounds. Biotechnol. Adv. 30 : 1524-1532.
24. Karabudak T., Bor M., Özdemir F., Türkan İ. 2014. Glycine betaine protects tomato (Solanum lycopersicum) plants at low temperature by inducing fatty acid de-saturase 7 and lipoxygenase gene expression. Mol. Biol. Rep. 41 : 1401-1410
25. Kopich V. N., Kretynin S. V., Kharchenko O. V., Litvi-novskaya R. P., Chashina N. M., Khripach V.A. 2010. Effect of 24-epibrassinolide on lipoxygenase activity in maize seedlings under cold stress. Biopolym. Cell. 3 : 218-224.
26. Kulkarni S., Das S., Funk C. 2002. Molecular basis of the specific subcellular localization of the C2-like domain of 5-lipoxygenase. J. Biol. Chem. 277 : 13167-13174.
27. Larkindale B. Huang A. 2004. Changes of lipid composition and saturation level in leaves and roots for heat-stressed and heat-acclimated creeping bentgrass (Agrostis stolonifera). Environ. Exp.Bot. 51 : 57-67.
28. Pinhero R.G., Paliyath G., Yada R.Y., Murr D.P. 1998. Modulation of phospholipase D and lipoxygenase activities during chilling. Relation to chilling tolerance of maize seedlings. Plant Physiol. Biochem. 36 : 213-224.
29. Lee S.H., Ahn S.J., Im Y.J., Cho K., Chung G.C., Cho B.H., Han O. 2005. Differential impact of low temperature on fatty acid unsaturation and lipoxygenase activity in figleaf gourd and cucumber roots. Biochem. Biophys. Res. Commun. 330 : 1194-1198.
30. Mao L. Panga H., Wangb G., Chenggang Zhuc C. 2007. Phospholipase D and lipoxygenase activity of cucumber fruit in response to chilling stress. Postharvest Biol. Technol. 44 : 42-47.
31. Meng K., Hou Y., Han Y, Ban Q., He Y., Suo J., Rao J. 2017. Exploring the functions of 9-lipoxygenase (DkLOX3) in ultrastructural changes and hormonal stress response during persimmon fruit storage. Int. J. Mol. Sci. 18 : 589-592.
32. Mosblech A., Feussner I., Heilmann I. 2009. Oxylipins: structurally diverse metabolites from fatty acid oxidation. Plant Physiol. Biochem. 47 : 511-517.
33. Porta H., Rocha-Sosa M. 2002. Plant lipoxygenases. Physiological and molecular features. Plant Physiol. 130 : 15-21.
34. Roy S., Maheshwari N, Chauhan R., Kumar Sen N. 2011. Structure prediction and functional characterization of secondary metabolite proteins of Ocimum. Bioinformation. 6 : 315-319.
35. Savchenko T.V., Zastrijnaja O.M., Klimov V.V. 2014. Oxylipins and plant abiotic stress resistance. Biochemistry (Mosc.). 79 : 362-375.
36. Stumpe M., Feussner I. 2006. Formation of oxylipins by CYP74 enzymes. Phytochem. Rev. 5 : 347-357.
37. Tiwari A., Avashthi H, Jha R., Srivastava A., Garg V., Pramod Ramteke W., Kumar A. 2016. Insights using the molecular model of lipoxygenase from finger millet (Eleusine coracana (L.)). Bioinformation. 12 : 156-164.
38. Van der Ent S., Van Wees S.C., Pieterse C.M. 2009. Jasmonate signaling in plant interactions with resistance-inducing beneficial microbe. Phytochem. 70 : 1581-1588

39. Wasternack C., Song S. 2017. Jasmonates: an update on bio-synthesis, metabolism, and signaling by proteins activating and repressing transcription. J. Exp. Bot. 68 : 1303-1321.