Visn. Hark. nac. agrar. univ., Ser. Biol., 2017, Issue 2 (41), с. 78-84


https://doi.org/10.35550/vbio2017.02.078




INDUCTION OF CALLUS FORMATION AND MICROCLONAL REPRODUCTION OF AMARANTHUS CAUDATUS L. (CV. HELIOS)


О. M. Yaroshko

Institute of Cell Biology and Genetic Engineering
of National Academy of Sciences of Ukraine
(Kyiv, Ukraine)
E-mail:
90tigeryaroshko90@gmail.com


The aim of the work was to induce the formation of callus tissue and microclones of Amaranthus caudatus L. the cv. Helios. The best medium for obtaining a stable callus culture was Murasige and Skoog (MS30) supplemented with 30 g/l of sucrose, 1 mg/l of 2,4-dichlorophenoxyacetic acid and 1 mg/l of kinetin; MS30 supplemented with 2 mg/l of 2,4-dichlorophenoxyacetic acid and 10% of coconut milk. For microclonal propagation the most effective medium was MS30 supplemented with 1 mg/l of thidiazuron.


Key words: Amaranthus caudatus, callus culture, micropropagation

 


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