Visn. Hark. nac. agrar. univ., Ser. Biol., 2017, Issue 2 (41), с. 41-47


https://doi.org/10.35550/vbio2017.02.041




POSSIBLE SIGNALING MEDIATORS OF INFLUENCE OF HYDROGEN SULPHIDE ON HEAT RESISTANCE OF PLANT CELLS

K. M. Firsova1, Yu. V. Karpets1, Yu. E. Kolupaev1, 2

1V. V. Dokuchaev Kharkiv National Agrarian University
(Kharkiv, Ukraine)

E-mail: plant_biology@ukr.net

2V. V. Karazin Kharkiv National University
(Kharkiv, Ukraine)


The possible reasons of intensifying of generation of reactive oxygen species (ROS) under the treatment of isolated wheat coleoptiles with the donor of hydrogen sulfide – 100 µM of NaHS – have been studied. In 2-4 h after treatment beginning there was approximately two times increase of endogenous content of hydrogen sulfide in coleoptiles, in 24 h the amount of H2S in them did not differ from control values. The effects of intensifying of generation of superoxide anion-radical and the increase of hydrogen peroxide content caused by sodium hydrosulfide in coleoptiles were leveled by pretreatment with NADPH-oxidase inhibitor diphenyleneiodonium chloride (DPI). At the same time butanol-1 (inhibitor of dependent on phospholipase D formation of phosphatidic acid, capable to activate NADPH-oxidase) did not exert the impact on H2S-induced generation of ROS in coleoptiles. The inhibitor of NADPH-oxidase DPI prevented with the development of heat resistance of coleoptile cells caused by the influence of hydrogen sulfide donor. Positive influence of hydrogen sulfide donor on heat resistance of wheat coleoptiles was leveled also by the treatment with inhibitor of phosphatidic acid formation butanol-1, but not with its biologically not active homologue butanol-2. It is supposed that phosphatidic acid as the signaling mediator can be involved in the realization of stress-protective effects of hydrogen sulfide; however it is not involved in the activation of ROS formation, dependent on NADPH-oxidase, by hydrogen sulfide in plant cells.


Key words: Triticum aestivum, hydrogen sulfide, reactive oxygen species, NADPH-oxidase, phosphatidic acid, heat resistance

 


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