Visn. Hark. nac. agrar. univ., Ser. Biol., 2017, Issue 2 (41), с. 32-40


https://doi.org/10.35550/vbio2017.02.032




EFFECT OF HYPERTHERMIA ON ACCUMULATION AND LOCALIZATION OF INDOLE-3-ACETIC ACID IN VARIETIES OF GLYCINE MAX (L.) MERR. DIFFERING ON RESISTANCE TO ABIOTIC STRESSORS

I. V. Kosakivska, L. V. Voytenko, K. M. Yarotska, R. V. Likhnyovskiy

M.G. Kholodny Institute of Botany
of National Academy of Sciences of Ukraine
(Kyiv, Ukraine)
E-mail:
phytohormonology@ukr.net


Effects of a short-term heat stress (40°С, 2 hours) on the content of free and conjugated indole-3-acetic acid (IAA) in the above-ground parts and roots of Glycine max (L.) Merr varieties, differing on resistance to abiotic stressors, were studied using the high performance liquid chromatography – mass-spectrometry method. In 35-day-old soybean plants, grown from inoculated with active strain of nitrogen-fixing bacterium Bradyrhizobium japonicum 634b and non inoculated seeds, free IAA acid was shown under control conditions to be dominant and its highest content was detected in roots with nitrogen-fixing nodules of the cold-tolerant variety Podilska 416. A non-specific response to hyperthermia was a decrease in endogenous IAA content in roots and above-ground parts of the two studied varieties. A specific response to the heat stress was free IAA dominance in roots that had no nodules, of the cold-tolerant variety Podilska 416, and conjugated IAA – in those of the drought-enduring variety KiVin. It was shown that auxin homeostasis in roots with nitrogen-fixing nodules of the hyperthermia-affected cold-resistant variety Podilska 416 was sustained by conjugation processes. The most pronounced changes in IAA content under hyperthermia conditions were observed in plants of the drought-enduring variety KiVin.


Key words: Glycine max, Bradyrhizobium japonicum, indolyl-3-acetic acid, hyperthermia

 


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